National Institute of Plant Genome Research
Digital India     
 
    Dr. Jagadis Gupta Kapuganti
    Staff Scientist IV
    Ph.D.: University of Wuerzburg, Germany
    Academic visitor: University of Oxford; Marie Curie Fellow, Oxford
    Postdoctoral Fellow: Max Planck Institute of Molecular Plant Physiology, Golm, Germany
    Tel: 91-11-26735111,14,17 Ext. - 111 :   Fax: 91-11-26741658
    Web: https://sites.google.com/site/jagadishguptapage/Home
    E-mail: jgk@nipgr.ac.in; jagadishguptak@gmail.com
 Career
Staff Scientist IV: National Institute for Plant Genome Research (Sep 2014-present)
Marie Curie Fellow: University of Oxford, UK (Sep 2012-Sep 2014)
Scientific staff: University of Rostock Germany (Nov 2010-Aug 2012)
Postdoctoral Fellow: Max Planck institute of Molecular Plant Physiology, Germany (Nov 2007-Nov 2010)
 Awards, Honours & Fellowships
2016: SERB Early Career Research Award
2015: Innovative Young Biotechnologist Award
2015: Ramanujan Fellowship from DST (not availed)
2014: Ramalingaswami Fellowship from DBT
2014: Hind Rattan Award NRI Welfare Society of India
2014: Young Investigator Meeting Travel Grant (India Bio Science)
2013: Listed in Marquis Who’s Who World 2013, 2014
2013: Plant Oxygen Group (International Free Radical Society travel fellowship winner)
2012: Presens OXYVIEW competition finalist
2012: Marie Curie Career Development Fellowship from European Union
2011: European Science Foundation: Short visit grant holder
2010: FEBS YTF travel grant
2007-2010: Postdoctoral Fellowship from German Research Foundation (DFG/SFB) at Max Planck Institute, Potsdam, Germany
2004-2007: Doctoral fellowship from German Research Foundation (DFG/SFB) in the research centre of mechanism of inter-specific interaction of organisms.
2004 January-2004 May: Short term fellowship from International Max Planck Research School, J   Jena, Germany
 Group Members
 

Dr. Alok Kumar Gupta
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Email: guptaalok53@gmail.com

 

 

Dr. Aakanksha Wany
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Email: awanynipgr@gmail.com

 

Dr. Abhay Pratap Vishwakarma
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Email: abhayps08@gmail.com

 

Ms. Sonika Pandey
NIPGR RA

 

 

Ms. Aprajita Kumari
IYBA-JRF

Email: aprajita786.roy@gmail.com

 

 

Mr. Vinod Kumar
SERB JRF

 

 

Mr. Pradeep Kumar Pathak
PhD Student

Email: pkpathak21@gmail.com

 

Ms. Pooja Singh
PhD Student

Email: pooestcute@gmail.com

 

Ms. Namrata Singh
PhD Student

Email: mennusingh@gmail.com

 

Rohit Kumar Manjhi
Lab Attendant

 

 Research
The functional role of nitric oxide in flooding stress:

Molecular oxygen is required for respiration in all aerobic organisms but oxygen levels are very low in some plant organs such as seeds, tubers and buds, where hypoxia is linked to quiescence. The release from hypoxia in these organs coincides with the transition of cells from the quiescent to the metabolically active state. In contrast, the hypoxia experienced by roots during flooding has deleterious effects on the tissues and can lead to cell death. Nitric oxide (NO), which is a powerful signalling molecule in plants and animals, is important in the release from seed dormancy and flooding tolerance but the mechanisms involved remain to be fully characterised. Our lab is currently working on to characterise the functions of NO in the adaptation to hypoxia and release from the hypoxic state, with a particular focus on the signalling functions of NO and reactive oxygen species (ROS) during the release of cells from the quiescent state in seeds and buds, and in roots subjected to flooding in the model plant species such as chickpea and tomato. Under oxygen limitation, regeneration of NAD+ for glycolysis is required for maintenance of a minimal ATP supply. Recent studies document that plants can fine tune oxygen consumption in response to low oxygen concentrations and maintain glycolysis by switching to alternative pathways for NAD+ regeneration, including nitrate reduction. The mechanisms that underlie this sensing and response to oxygen deprivation have not been fully elucidated. It is also not clear whether plants use NO as a major regulator of bioenergetics and oxygen homeostasis as animals are known to do. At present answering these questions is very important priority of our lab in order to achieve complete understanding of low oxygen sensing mechanisms and the metabolic adaptations to oxygen deprivation in plants. Our research in this line will provide new insights into these mechanisms and the metabolic adjustments associated with controlled use of carbohydrate and ATP during hypoxic survival. The knowledge gained from this project will help to develop plants with better survival and performance in areas suffering from frequent rainstorms and flooding. 

Role of nitrogen nutrition on plant pathogen Interactions:

Plants need nitrogen because it’s a major constituent of the plants. Nitrate and ammonium are the major N sources for the plants. Biological mineralization (complex chemical reactions) provides nitrate or ammonium to the plants. The application of nitrogen (N) fertilizer has been a major factor in modern high crop yields and must play a major role in meeting the food security agenda.  Ammonium as a N form of nitrogen enters into the soil via fixation of atmosphere nitrogen; decomposition of organic matter by bacteria and fungi, By nitrification pathway ammonium is oxidized to nitrite and further nitrate. Nitrate is a component of many primary and secondary metabolites. Pathogen attack represents a significant source of reduced crop yield, so that optimizing plant resistance to disease is equally important. In order to meet the challenge of allowing the maximizing the benefits of N fertiliser use whilst minimizing the effects of increased pathogen-susceptibility, We are investigating effect of N nutrition on plant resistance to pathogens. Our ongoing affords to generate various transgenic plants that have altered nitrate reductase activity, using these plant we will further investigate nitrate nutrition role under biotic stress. Most importantly we will examine examine N-flux in the plant and metabolomic analyses of plant-microbial exchanges in the apoplast to better understand how N type can influence pathogen virulence and plant resistance.  To determine how application of the non-pathogenic biocontrol fungus Trichoderma could increase both resistance and the efficiency of the use of certain types of fertilise

Maximizing nitrogen use efficiency (NUE) in crops by understanding a nitric oxide-mediated modulatory switch

Our lab in collaboration with IBERS, UK, will explore a novel method of increasing nitrogen use efficiency (NUE) in crops to help meet the food security agenda. Nitric oxide is a product of nitrogen (N) metabolism but excess of NO production may suppress N uptake thereby acting as a negative feedback mechanism. Intrinsic to this feedback mechanism is the-suppression of nonsymbiotic haemoglobin (snHb) expression which would otherwise oxidize the excess of NO to NO3. In this context we will establish how snHb expression is regulated to modulate NO accumulation and further we will explore how NO modulates N-channels and NUE.



 Publications from NIPGR
Pandey S, Kumari, Gupta KJ: Measurement of respiration and internal oxygen in germinating Cicer arietinum L. seeds using micro sensor. Methods in Molecular Biology (In Press)
Kumari A and Gupta KJ: VisiSens technique to measure internal oxygen and respiration in barley roots. Methods in Molecular Biology (In Press)
Gupta KJ, Lee CP, Ratcliffe RG: Nitrite Protects Mitochondrial Structure and Function under Hypoxia.Plant and Cell Physiology (Press)
Mur L.A.J., Simpson C., Kumari A.., Gupta A.K., Gupta K.J.  Moving nitrogen to the centre of plant defence against pathogens. Annals of Botany (Press)
Wany A.,  Gupta A.K., Kumari A., Gupta S., Mishra S.,  Jaintu R., Pathak P.K., Gupta  K.J. (2016) Chemiluminescence detection of nitric oxide from roots, leaves and root mitochondria. Methods in Molecular Biology 424:15-29.
Kumari A., Wany A., Mishra S., Gupta A.K., Gupta K.J. (2016) Nitric oxide measurement from purified enzymes and estimation of scavenging activity by gas phase chemiluminescence. Methods in Molecular Biology 1424:31-8.
Gupta A.K., Kumari A., Mishra S., Wany A., and Gupta K.J. (2016) The functional role of nitric oxide in plant mitochondrial metabolism. Advances in Botanical Research 77: 145-163.
Wany A., and Gupta K.J. (2016) Localization of nitric oxide in wheat roots by DAF fluorescence. Methods in Molecular Biology 1424:39-47
Royo B., Moran J.F., Ratcliffe R.G., Gupta K.J. (2015) Nitric oxide induces the alternative oxidase pathway in Arabidopsis seedlings deprived of inorganic phosphate.Journal of Experimental Botany66: 6273-80
 Previous Publications
Horn R., Gupta K.J., Colomobo N (2014) Mitochondrion role in molecular basis of cytoplasmic male sterility. Mitochondrion 19:198-205.
Gupta K.J., Mur L.A.J., Ratcliffe R.G. (2014) Guarding the guard cells? New Phytologist. 203: 349-351.
Gupta KJ., Brotman Y., Mur L.A.J. (2014) Localisation and quantification of reactive oxygen species and nitric oxide in Arabidopsis roots in response to fungal infection. Bio Protocols (Online).
Gupta K.J., Hebelstrup K.H., Kruger N.J., Ratcliffe R.G. (2014) Nitric oxide is required for homeostasis of oxygen and reactive oxygen species in barley roots under aerobic conditions. Molecular Plant 7:747-750.
Gupta K.J., Mur L., Brotman Y. (2014) Trichoderma asperelloides suppresses nitric oxide generation elicited by Fusarium oxysporum in Arabidopsis roots. Molecular Plant-Microbe Interactions. 27: 307-314.
Gupta K.J and Ewald R (2015) Simultaneous isolation of root and leaf mitochondria from Arabidopsis. Alternative respiratory pathways in higher plants. In: K.J. Gupta., L.A.J. Mur., B. Neelwarne (Editors). John Wiley and Sons, Oxford. pp 359-365..
Gupta KJ., and Igamberdiev A.U. (2014) Compartmentalization of reactive oxygen species and nitric oxide production in plant cells – an overview. pp 1-14. Springer Verlag New York.
Gupta K.J and Igamberdiev A.U. (2014) Reactive Oxygen and Nitrogen Species Signaling and Communication in Plants. In: Gupta K.J, Igamberdiev A.U. (Eds). Springer Verlag. New York.
Gupta K.J., Neelwarne B., Mur L.A.J. (2015) Integrating classical and alternative pathways. In Alternative respiratory pathways in higher plants. In:  K.J. Gupta, L.A.J. Mur, B. Neelwarne (Editors). John Wiley and Sons, Oxford 1-26
Mur L.A.J., Gupta K.J., Chakraborty U., Chakraborty B., Hebelstrup K.H. (2015) Ethylene, nitric oxide and haemoglobins in plant tolerance to flooding. Abiotic Stresses in Crop Plants, pp 43.
Igamberdiev A.U., Ratcliffe R.G., Gupta K.J. (2014). Plant mitochondria: Source and target for nitric oxide. Mitochondrion. 19:329-333.
Gupta K.J., Brotman Y., Segu S., Zeier T., Zeier J., Persijn S.T., Cristescu S.M., Harren F.J.M., Bauwe H., Fernie A.R. (2013) The form of nitrogen nutrition affects resistance against Pseudomonas syringae pv. phaseolicola in tobacco. Journal of Experimental Botany (64): 553-568.
Gupta K.J., and Igamberdiev A.U. (2013) Recommendations of using at least two different methods for measuring NO. Frontiers in Plant Science 4
Mur L.A.J., Hebelstrup K.H., Gupta K.J. (2013) Striking a balance: does nitrate uptake and metabolism regulate both NO generation and scavenging? Frontiers in Plant Science 4:288.
Mur L.A.J., Mandon J., Persijn S., Cristescu S.M., Moshkov I.E., Novikova G.V., Hall M.A., Harren F.J.M., Hebelstrup K.H., Gupta K.J. (2013) Nitric oxide in plants: an assessment of the current state of knowledge. AoB Plants 5.
Gupta K.J., Shah J.K., Brotman Y., Jahnke K., Willmitzer L., Kaiser W.M., Bauwe H., Igamberdiev A.U. (2012) Inhibition of aconitase by nitric oxide leads to induction of the alternative oxidase and to a shift of metabolism towards biosynthesis of amino acids. Journal of Experimental Botany 963: 1773-1784..
Gupta K.J., Igamberdiev A.U., Mur L.A.J. (2012) NO and ROS homeostasis in mitochondria: a central role for alternative oxidase. New Phytologist 195: 1-3.
Gupta K.J., and Rolletschek H. (2012) Plant respiratory metabolism: a special focus on the physiology of beetroot (Beta vulgaris L.) mitochondria. Red Beet Biotechnology, pp. 91-104. Springer US.
Manjunatha G., Gupta K.J., Lokesh V., Mur L.A., Neelwarne B. (2012) Nitric oxide counters ethylene effects on ripening fruits. Plant Signal Behaviour 7: 476-483.
Gupta K.J., HebelstrupK.H., Mur L.A.J., Igamberdiev A.U. (2011) Plant hemoglobins: important players at the crossroads between oxygen and nitric oxide. FEBS letters 585: 3843-3849.
Engel N., Ewald R., Gupta K.J., Zrenner R., Hagemann M., Bauwe H. (2011) The pre sequence of Arabidopsis serine hydroxymethyltransferase SHM2 selectively prevents import into mesophyll mitochondria. Plant Physiology 157: 1711-1720.
Ramirez-Aguilar S.J., Keuthe M., Rocha M., Fedyaev V.V., Kramp K., Gupta K.J., Rasmusson A.G., Schulze W.X., van Dongen J.T. (2011). The composition of plant mitochondrial super complexes changes with oxygen availability. Journal of Biol Chem 286: 43045-43053.
Gupta K.J. (2011) Protein S-nitrosylation in plants: photorespiratory metabolism and NO signaling. Science Signaling 154 jc1.
Gupta K.J., Fernie A.R., Kaiser W.M., van Dongen J.T. (2011) On the origins of nitric oxide. Trends in Plant Science16:160-168.
Van Dongen JT, Gupta K.J., Ramírez-Aguilar SJ, Araujo WJ, Nunes-Nesi A, Fernie AR (2011) Regulation of respiration in plants: A role for alternative metabolic pathways. Journal of Plant Physiology 168:1434-1443.
Gupta K.J., Hincha D.K., Mur L.A.J. (2011) NO way to treat a cold. New Phytologist. 189:360-363..
Manjunatha G., Lokesh S.V., Neelwarne B., Singh Z., Gupta K.J. (2011) Nitric oxide applications for quality enhancement of horticulture produce. Horticulture reviews 42.
Gupta K.J., Igamberdiev A.U. (2011) The anoxic mitochondrion as nitrite: NO reductase. Mitochondrion 11: 537-543.
Gupta K.J., Igamberdiev A.U., Manjunatha G., Segu S., Moran J.F., Neelawarne B., Bauwe H., Kaiser W.M. (2011) The emerging roles of nitric oxide (NO) in plant mitochondria. Plant Science181: 520-6.
Gupta K.J., Bauwe H., Mur L.A.J. (2011) Nitric Oxide, Nitrate Reductase and UV-B tolerance. Tree Physiology 31: 795–797.
Brotman Y., Gupta K.J., Vetribo A. (2010) Trichoderma: Quick Guide. Current Biology 20: 390-391.
Mur L.A.J., Lloyed A.J., Gupta K.J., Chakraborty U., Chakraborty B. (2014) Harnessing the power of metabolomics to improve crop tolerance against stress. Review of Plant Pathology. 6 (59-92) Scientific Publishers. ISBN: 978-81-7233-916-6.
Gupta K.J., and Kaiser W.M. (2010) Production and scavenging of nitric oxide by barley root mitochondria. Plant and Cell Physiology 51: 576-584.
Gupta K.J., and Igamberdiev A.U. (2010) Kudzu invasion leads to NOx increase and ozone pollution: unravelling possible mechanisms. Proceedings of the National Academy of Sciences (USA) 107: E 153..
Gupta K.J., Igamberdiev A.U., Kaiser W.M. (2010) New insights into the mitochondrial nitric oxide production pathways. Plant Signalling and Behaviour. 5: 999–1001.
Zabalza A., van Dongen J.T., Froehlich A., Oliver S., Faix B., Gupta K.J., Schmalzlin E., Igal M., Orcaray L., Royuela M., Geigenberger P. (2009) Regulation of respiration and fermentation to control plant internal oxygen concentration. Plant Physiology 149: 1087-1098.
Rumer S., Gupta K.J., Kaiser W.M. (2009) Plant cells oxidize hydroxylamines to NO. Journal of Experimental Botany 60: 2065-72.
Gupta K.J., Zabalza A., van Dongen J.T. (2009) Regulation of respiration when the oxygen availability changes. Plant   Physiology 137: 383-391.
Rümer S., Gupta K.J., Kaiser W.M. (2009) Oxidation of hydroxylamine to NO by plant cells. Plant Signalling and Behaviour 4: 853-855.
Stoimenova M., Igamberdev A.U., Gupta K.J., Hill R.D. (2007) Nitrite driven anaerobic ATP synthesis by barley and rice root mitochondria. Planta 226: 465-474.
Kaiser W.M., Gupta K.J., Planchet E. (2007) Higher plant mitochondria as source of NO. Plant Cell monographs1-11. In: L Lamattina, JC Polacco, eds, Nitric Oxide in Plant Growth Springer, Berlin.
Planchet E., Gupta K.J., Sonoda M., Kaiser W.M. (2005) Nitric oxide emission from  tobacco leaves and cell suspensions: rate-limiting factors and evidence for the involvement of mitochondrial electron transport. The Plant Journal 41: 732–743..
Gupta K.J., Stoimenova M., Kaiser W.M. (2005) In higher plants, only root mitochondria, but not leaf mitochondria reduce nitrite to NO, in vitro and in situ. Journal of Experimental Botany 56: 2601-9.
 Books
Gupta K.J., Igamberdiev A.U. (Editors) (2015) Reactive Oxygen and Nitrogen Species Signaling and Communication in Plants. Springer, Switzerland 2015..
Gupta K.J., Mur, L.A.J., Neelwarne B. (Editors) (2015). Alternative respiratory pathways in higher plants. John Wiley and Sons, Oxford 2015.
Gupta KJ (Editor) (2016): Plant nitric oxide: Methods & protocols: Methods in Molecular Biology, Humana Press. USA 2016.